Comprehensive Identification of Reliable Reference Genes for qRT-PCR Normalization of Fusarium oxysporum-Resistant Genes’ Expressions in Lilium sargentiae Wilson

Fusarium wilt (caused by oxysporum f. sp. Lilii) is one of the most damaging diseases in lily (Lilium sargentiae Wilson). Although some F. oxysporum-resistant varieties have been identified and are being utilized resistant breeding, regulation network resistance-associated mechanisms yet to be studied due lack reliable reference genes for qRT-PCR (quantitative reverse transcription PCR) normalization. The reliability results relies mainly on stability genes. This study investigated nine candidate (CYP, EF1-α, GAPDH, TUB, UBQ, AQP, HIS, PGK, RPL13) analysis Expression via common programs GeNorm, BestKeeper, NormFinder, at different time points post-inoculation oxysporum, revealed that all met basic requirements Amongst them, HIS GAPDH displayed highest lowest expression stability, respectively. was further validated analyzing levels four resistance-related patterns target were consistent with their responses pathogenetic fungi other plants. Our show suitable gene accurately normalizing genes’ expressions L. sargentiae.